Reticulocyte Dye yeSysmex Hematology Analyzers inopa yakanangwa fluorescent nucleic acid staining ye semiconductor laser flow cytometry mapuratifomu. Iyo mvura reagent inopinda nucleated masero eropa, reticulocytes, uye maplatelet kuti abatanidze zvakasarudzwa ne intracellular RNA uye DNA, ichigadzira yakananga divi fluorescence uye masaini ekuparadzira masaini munzira dzakatarwa dzekuona. Yakagadzirwa kuti ibatane zvakananga neautomated fluidic masisitimu, mhinduro inochengetedza kugadzikana kwefluorescence intensity, ruzha rwekumashure, uye zvakawanda zvinogoneka-ku{3}}kushanda kwakawanda muzvirongwa zverabhoritari yekiriniki.
Chii chinonzi Hematology Stain / Dye Reagents?
Automated hematology dye reagents imagetsi efluorescent akagadzirirwa kuyerera-cytometry-based cell analysis platforms. Kusiyana nemaitiro echinyakare eropa smear tsvina anoshandiswa muchiedza microscope, aya emvura madhayi anopinda mumasero anotariswa kuburikidza neanodzorwa membrane permeabilization kuti anyore intracellular nucleic acids. Pasi pe semiconductor laser excitation mukati me optical flow cell, ma cellular structures akanyorwa anoburitsa side fluorescence uye light scatter masiginecha, zvichibvumira masystem optics kuburitsa chaiwo maviri-dimensional scattergrams uye sero cluster kuparadzirwa.
Basa muKuronga Kwesero / Kuonekwa
Mukuongorora hematology yeOptical, madhayi efluorescent anogonesa kusiyanisa masero nekuenzanisa intracellular nucleic acid density:
Musiyano weWhite Blood Cell:Inoparadzanisa lymphocytes, monocytes, neutrophils, eosinophils, uye basophils zvichienderana neRNA / DNA ratios uye cytoplasmic structure.
Atypical & Immature Cell Identification:Inozivisa kuputika maseru uye immature granulocytes kuburikidza neyakakwirisa fluorescence emission intensity.
Reticulocyte Kuverenga:Inoyera yasara ribosomal RNA mudiki masero matsvuku eropa kuongorora bone marrow erythropoietic chiitiko.
Optical Platelet Kuverenga:Inogadzirisa giant maplatelet uye inosiyanisa zvidimbu zvemasero matsvuku eropa kubva kumaplatelet uchishandisa chinangwa nucleic acid fluorescence gating.
Detection Channel
Iyo dhayi yefluorescent inoshanda pane chaiyo otomatiki optical chiteshi:
WNR Channel:Inoverenga masero machena eropa uye inoparadzanisa nucleated masero matsvuku eropa kuburikidza nekusiyanisa nucleic acid staining.
WDF Channel:Inosiyanisa mature leukocyte subpopulations uye inoona immature granulocytes uchishandisa divi fluorescence uye divi kuparadzira paramita.
WPC Channel:Masikirini emasero ekutanga uye abnormal lymphocytes anoenderana nenyukireya density uye musiyano membrane permeability.
RET Channel:Inoyera zvikamu zve reticulocyte uye optical red blood cell parameters kuburikidza neRNA fluorescence intensity.
PLT Channel:Inonyora platelet nucleic acids kubvisa microcytic red blood cell interference panguva yekuverenga optical.
Inoenderana Analyzer Series
Yakagadzirirwa kurongeka kwekushanda kwakananga neatomatiki hematology mapuratifomu anoshandisa semiconductor laser flow cytometry:
XN Series:XN-1000, XN-2000, XN-3000, XN-9000, XN-L Series (XN-350, XN-450, XN-550)
XS Series:XS-500i, XS-800i, XS-1000i
XT Series:XT-1800i, XT-2000i, XT-4000i
XE Series:XE-2100, XE-5000
Zvigadzirwa Zvinotsanangurwa
|
Parameter |
Kutsanangurwa Kwehunyanzvi |
|
Chimiro Chemuviri |
Mushonga wemvura, wakajeka kusvika kuchiedza chero |
|
Excitation Wavelength |
633 nm / 635 nm |
|
Emission Wavelength |
650 nm - 670 nm |
|
Target Binding |
Ribonucleic Acid (RNA) uye Deoxyribonucleic Acid (DNA) |
|
Zvakawanda-ku-Zvizhinji zveCV |
< 2.0% (SFL channel) |
|
Particulate Sefa |
0.2 µm mhedziso membrane yakasefa |
|
pH Range |
7.20 - 7.50 pa25 degree |
|
Osmolality |
280 - 320 mOsm/kg |
|
Quality Standard |
ISO 13485 / CE IVD inoenderana |
Kushandisa & Reagent Basa
WNR Channel Staining (STAC-DYE-SYSWNR):Inogonesa maseru membranes kuti ibvumidze kukurumidza kupinda dhayi yenyukireya, ichipatsanura yakasimba NRBC nuclei kubva kumasero machena eropa kune chaiyo NRBC% uye WBC kuverenga chizvarwa.
WDF Channel Staining (STAC-DYE-SYSWDF):Stains intracellular nucleic acids yeleukocytes kuti ibudise masumbu e scattergram akasiyana neutrophils, lymphocytes, monocytes, uye eosinophils.
WPC Channel Staining (STAC-DYE-SYSWPC):Sarudzo inoshatisa kuputika maseru uye abnormal lymphocytes yakavakirwa lipid kuumbwa uye nyukireya density kune yechipiri sero mureza.
RET Channel Staining (STAC-DYE-SYSRET):Inosungira kune yakasara RNA mumasero matsvuku eropa, zvichiita kuti kuverenga kwereticulocyte percentage, absolute count, uye immature reticulocyte chikamu.
PLT Channel Staining (STAC{{0}DYE-SYSPLT):Inoisa ma platelet nucleic acids kudzivirira kukanganisa kwe microcytic red blood cell panguva yakaderera{0}count optical platelet measurements.
Package / Storage
Packaging Configurations:
- Makaseti eCartridge: 2 × 12 mL
- Mhinduro yemubhodhoro: 1 × 50 mL, 1 × 100 mL, 1 × 500 mL
Kuchengetedza Tembiricha:2 dhigirii kusvika 30 dhigirii (Chengetedza kubva kuchiedza chakananga uye nechando)
Hupenyu Husina Kuvhurwa:18 mwedzi
Pa-Kugadzikana Kwebhodhi:Mazuva makumi matanhatu mumamiriro emurabhoritari ambient
Related Hematology Reagents
Hematology Diluent Solutions:Isotonic buffer mhinduro dzinochengetedza sero vhoriyamu kutendeseka uye kudzora fluid conductivity panguva yekuyera kwemuviri.
Lyse Reagents yeLeukocyte Kusiyana:Active mhinduro dzinosarudza lyse masero matsvuku eropa kugadzira masero machena eropa kuongororwa kwemaziso.
Analyzer Yekuchenesa Solutions:Enzymatic yekugeza zvinwiwa zvakagadzirirwa kujekesa mapuroteni anovaka uye dhayi zvakasara kubva mukati fluidic mitsetse uye optical kuyerera maseru.
Concentrated Sheath Fluids:Zvinwiwa zveHydrodynamic zvakagadzirirwa kuisa pakati sampuli hova mukati memakamuri ekuona maziso.
FAQ
Mubvunzo: Dhayi iyi inodzivirira sei kupindirana kwechiratidzo pakati pe reticulocytes nemasero matsvuku eropa akakura?
A: Iyo dhayi inotarisa RNA tambo dziripo mune reticulocytes. Maseru matsvuku eropa akakura haana inoshanda RNA kana DNA, isingabudisi kumashure fluorescence pasi pelaser excitation, izvo zvinogadzira kupatsanurwa kwakasiyana kwekutanga pane optical scattergrams.
Mubvunzo: Ndeipi iyo yakavhurika{0}vial kugadzikana kweiyo reagent kana yaiswa muchiridzwa?
A: Magadzirirwo emvura anoramba akatsiga pa-pabhodhi kwemazuva anosvika 60 ari pakati pe2℃ne30℃. Kudzikamisa vamiririri vanodzivirira kutenderera kwekuchinja uye kuchengetedza inogara iine fluorescence kuburitsa mukati menguva yese yekushandisa.
Mubvunzo: Hardware kana software inogadziriswa inodiwa kana uchiunza iyi dhayi reagent?
A: The reagent inofanana ne-chemical parameters, excitation response curves, uye fluidic viscosity, zvichibvumira kushanda kwakananga pasi pezvirongwa zvekugadzirisa masisitimu.
Mubvunzo: Kuumbwa kwemvura kunodzivirira sei masero ekuyerera kubva pakuunganidzwa kwechikamu?
A: Mhinduro dzinogadziriswa kuburikidza ne0.2 µm absolute membrane mafirita panguva yekupedzisira kurongedza. Kusaita-kusanganiswa kwedhayi chimiro kunodzivirira kuumbwa kwemavara madiki-nezvisaririra mukati memitsetse inoyerera nemhuno.
Q: Ndeapi magwaro anoperekedza batch kutumira kune yehuhu ongororo?
A: Kuendeswa kunosanganisira Chitupa cheOngororo (CoA), Safety Data Sheet (SDS), marekodhi ekuongorora mijenya, uye ISO 13485 kutevedzera zvinyorwa.
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